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Essay: Gene transfer techniques – pros and cons. Gene transfer is Gift Essay, a technique to on Relationships Essay example stably and efficiently introduce functional genes (that are usually cloned) into the target cells. Genetic transfer is the mechanism by which DNA is transferred from a donor to a recipient. Once donor DNA is inside the recipient, crossing over can occur. The result is a recombinant cell that has a genome different from either the donor or the recipient. A recombination event must occur after transfer in order that the change in the genome be heritable (passed on to the next generation).The genes are the blueprints essential to generate all the Gift to Humans Essay proteins in our bodies which eventually perform all the biological functions. Therefore, when a gene is Studies Essay example, efficiently introduced into Culture is a Gift a target cell of the host, the protein which is Age Coined by Mark and Charles Dudley Warner, encoded by that gene is produced . Gene transfer technologies developed initially as a research tool for studying the gene expression and its function.
A range of is a Gift techniques and naturally occurring processes are utilized for the gene transfer. Techniques are available for Essay the introduction of DNA into Culture Essay many different cell types in culture, either to Gilded Mark and Charles Dudley Essay study gene function and regulation or to produce large amounts of recombinant protein. Cell cultures have therefore been used on Essay, a commercial scale to synthesize products such as antibodies, hormones, growth factors, cytokines and viral coat proteins for immunization.The most important application of this technology is in of the are consumer durable vivo gene therapy, i.e. the introduction of DNA into the cells of live animals in order to treat disease. The extraordinary potential of altered DNA molecule is to give rise to new life-forms that are better adopted for survival. Change in base sequence of DNA leads to change in Gift Essay protein causing disease condition which can be corrected by manipulation of gene. Much emphasis has been given to Essay on Concurrent Disoders the gene manipulation in cardiovascular diseases, parkinson’s disease, lysosomal disorders, ocular gene therapy and osteoarthritis. Overview of gene-transfer strategies: Gene transfer can be achieved essentially via three routes. The most straightforward is is a to Humans Essay, direct DNA transfer, the physical introduction of foreign DNA directly into thomas essay the cell.
For example, in cultured cells this can be done by microinjection, whereas for is a Gift cells in Age Coined Twain Dudley Essay vivo direct transfer is often achieved by bombardment with tiny DNA-coated metal particles. The second route is termed transfection, and this encompasses a number of techniques, some chemical and some physical, which can be used to persuade cells to take up DNA from their surroundings. Culture To Humans? The third is to package the DNA inside an. animal virus, since viruses have evolved mechanisms to naturally infect cells and introduce their own nucleic acid. The transfer of foreign DNA into a cell by this route is termed transduction. The choice of methods of DNA transfer depends upon the target cells in on Relationships which transformation will be performed.
It also depends upon the objectives of Culture to Humans gene manipulation. The transfection may be either stable or transient. Although, choice of oryx quotes DNA transfer method is very important, the is a to Humans Essay other important steps are selection of gene, isolation of gene, preparing recombinant DNA and selection of transformed cells. The regeneration of organism with new characteristics is also equally important. The delivery of genes in vitro can be done by treating the cells with viruses such as retrovirus or adenovirus, calcium phosphate, liposomes, particle bombardment, fine needle naked DNA injection, electroporation or any combination of these methods. These are the powerful tools for research and have possible applications in gene therapy. FACTORS TO CONSIDER WHEN SELECTING A GENE DELIVERY METHOD. The advancement of new technologies has made gene delivery both more efficient yet more confusing given the formidable array of reagents and methods now available. Thomas? Determining the best gene delivery approach for any particular experiment requires careful consideration of a number of different factors as stated below. 1. Cellular Context or Environment – The context or environment of the Gift to Humans cells (i.e., in vivo vs. in.
vitro vs. Gilded Age Coined Twain Dudley Warner? ex vivo) to which nucleic acids will be delivered is the first factor to consider when. deciding upon a gene transfer method. The obstacles and challenges posed by Gift Essay delivery to in. vitro cell cultures are very different than those posed by in vivo or ex vivo cells, and require. different tools and Essay on Concurrent Disoders, techniques for overcoming them. For example, targeting nucleic acids to. particular cells while avoiding other cells is usually a major concern for in vivo gene transfer.
applications, sometimes a concern for ex vivo applications, but usually not a concern for in vitro. Another important issue that must be addressed when planning in vivo (but not in vitro) gene. delivery studies is the immunogenicity of the gene transfer system. This is particularly important when considering the use of certain viral vectors discussed previously, which may be highly immunogenic. Also related to immunogenicity is the issue of Culture is a Gift to Humans Essay general safety, both for the experimenter and for the subject of any study utilizing transfection. Age Coined By Mark And Charles? Almost always, the to Humans Essay use of viral vectors requires very extensive safety precautions than does the use of synthetic transfection methods. 2. Cell or Tissue Type – The origin of the cells or tissue to which nucleic acids will be.
transferred is typically the second parameter considered when selecting a gene delivery. method. For a number of different reasons, the difficulty of cellular nucleic acid delivery varies. from cell type to cell type. Because of this, it is always important to review the literature to. determine if the cell type of oryx and crake quotes interest has previously been utilized for gene transfer experiments, which exact methods have been used, and what delivery efficiencies were achieved, i.e., what. percentages of cells actually transfected or transduced, with each method.
Certain cell types are often described as ‘notoriously difficult to transfect’. These include primary cell cultures of many varieties. Successful gene transfer to Gift such cells. usually requires a good deal of Disoders research before selecting any particular gene delivery method. 3. Gift To Humans Essay? Delivery Efficiency – For most applications, transfecting or transducing as many cells as.
possible is preferred, especially when it is necessary to detect the presence of rare transgene. products. Thus, it is Essay example, important to determine the level of delivery efficiency required for a given. application before deciding on a particular gene transfer method. In most cases, it is Culture is a Gift, found that. viral vectors provide the highest delivery efficiencies. And Crake Quotes? For some commonly used and easily transfected cell lines, cationic lipids nearly match viral vectors in their gene transfer efficiency, while providing safer and much quicker procedures.
4. Stable vs. Culture Is A? Transient Gene Delivery – Gene transfer procedures are frequently categorized. by whether the delivered gene remains separate from the Studies on Relationships example host cell chromosome or whether it is integrated into the host cell chromosome. In the first case, known as transient gene delivery, expression of the transgene typically dissipates after a given period of time – usually within. several days – because the expression vector is either degraded or expelled from the host cell. In the second case, known as stable gene delivery, expression of the is a Gift to Humans transferred gene is.
prolonged, or stable, because the vector is integrated into the host cell chromosome. Obviously, different applications require different time periods of transgene expression. Thus, a careful. comparison between different gene delivery methodologies will allow a suitable choice for. generating the desired transient or stable expression.
In the case of viral vectors, determining whether a particular virus is thomas, suited for stable or transient. transduction is Culture Gift, simply a matter of jefferson learning the general characteristics of the vector. Gift To Humans? In the Studies case. of chemical or physical/mechanical gene transfer, determining whether a given method is. suitable for transient vs. stable delivery may be as easy as reading sales literature from the. manufacturer or checking primary research literature.
5. Type of Delivered Molecule – Another factor to consider when selecting a gene delivery. method is what type of nucleic acid or other molecule is being transfected or transduced. For. example, a reagent that efficiently delivers plasmids may not efficiently deliver DNA. oligonucleotides. It is Gift to Humans Essay, frequently easiest to oryx and crake quotes simply review the literature to Culture is a Gift learn what methods have been used successfully, and to contact other researchers who have experience delivering the molecule of The Pros and Cons interest. 6. Cytotoxicity – Another issue that comes up frequently when choosing among different gene. delivery methods is the is a to Humans Essay cytotoxicity of the various methods and reagents. Some methods, such.
as electroporation and gene guns, can be extremely harsh on cells and often result in Essay on Concurrent the death. of a majority of cells. Also, the Culture is a Gift to Humans DEAE-dextran can be quite harsh on some cell types, especially. when a DMSO or glycerol shock step is used as part of the procedure. In some applications, such as when a small number of transfectants are required from a recalcitrant cell type, this is.
not a problem. In the majority of The Pros applications however, a high percentage of cell death is simply. not acceptable. The plethora of available cationic lipids and polymer reagents have varying. degrees of toxicity with different cell types, and their concentrations during transfection can be. optimized as needed to minimize cell death. 7. Suspension vs. Adherent cells – Most commonly transfected cells grow – and are. transfected – while attached to some type of Culture is a to Humans Essay support, whether that support is the tissue from. which they are derived, some inert membrane or scaffolding, or the Age Coined Mark Twain Essay surface of a tissue culture. plate.
Less common are cells that grow while suspended in medium, such as those derived from. blood and other bodily fluids. Suspension cells are generally more difficult to transfect; therefore, it may be necessary to rely on harsher gene delivery methods, such as. electroporation, gene guns, microinjection, or viral vectors for acceptable transfection levels. Also, some cationic lipids can effectively transfect suspension cells, and should be tried when. possible due to their simplicity and cost-effectiveness. 8. Expertise – The degree of difficulty in performing the various transfection techniques can. vary significantly, and therefore the expertise and experience of the researcher is usually an. important factor to consider. For example, methods like microinjection viral transfection methods are invariably more complex than cationic lipids and polymer reagents. Is A Essay? Also, of the Twain and Charles Dudley Essay chemical methods, calcium phosphate co-precipitation can be quite tricky due to its sensitivity to slight changes in experimental conditions.
9. Time – Another important factor to consider is the time required for gene delivery. Viral. methods, while highly efficient, can be quite time consuming, typically taking anywhere from two weeks to Culture is a one month to complete. In contrast, chemical and physical/mechanical methods can usually be completed within a few days, but frequently provide lower transfection efficiencies. Thus, it is not uncommon to have to weigh the benefits of faster results and less labor against the benefit of higher efficiency. 10 Cost – Finally, cost can be a major factor when deciding on the best gene delivery.
method for a given application. The most expensive methods tend to be the. physical/mechanical methods, such as electroporation and gene guns, due to the specialized. equipment they require.Viral gene delivery is perhaps the second most expensive method, since it requires the which durable goods? vector reagents, extensive time and labor to complete lengthy vector preparation procedures, as well as proper disposal of viral waste materials. The third most expensive gene delivery methods utilize polymers and cationic lipids, which do not require any specialized equipment or lengthy viral vector preparation steps. Such reagents typically cost in the hundreds of dollars, depending on the number of transfections to be completed.
Finally, the least expensive methods utilize calcium phosphate, which is a cheap and abundant mineral, and Culture Essay, naked DNA, which obviously requires no transfection reagent cost, but can be highly inefficient. GENE TRANSFER TECHNIQUES. Transformation is the naturally occurring process of gene transfer which involves absorption of the genetic material by a cell through cell membrane causing the fusion of the foreign DNA with the and crake quotes native DNA resulting in Culture Essay the genetic expression of the received DNA. Transformation involves the uptake of “naked” DNA (DNA not incorporated into structures such as chromosomes) by and Cons Essay competent bacterial cells. Culture Is A Gift To Humans? Cells are only competent (capable of taking up DNA) at a certain stage of their life cycle, apparently prior to jefferson essay the completion of cell wall synthesis . Transformation is usually a natural method of gene transfer but as a result of Gift Essay technological advancement originated the artificial or induced transformation. Thus there are two types called as natural transformation and artificial or induced transformation. In natural transformation, the foreign DNA attaches itself to the host cell DNA receptor and with the help of the protein DNA translocase it enters the host cell. Gilded By Dudley Essay? The presence of nucleases restricts the entry of two strands of the Culture is a Essay DNA, destroys a single strand thus allowing only one strand to enter the host cell. Any DNA that is not integrated into the chromosome will be degraded. This single stranded DNA mingles with the host genetic material successfully.
The artificial or induced method of thomas jefferson transformation is done under laboratory condition which is Culture is a Gift to Humans, either a chemical mediated gene transfer or done by electroporation.. Genetic engineers are able to induce competency by thomas essay putting cells in certain solutions, typically containing calcium salts. At the entry site, endonucleases cut the Culture is a Gift Essay DNA into fragments of 7,000-10,000 nucleotides, and the double-stranded DNA separates into and crake single strands. The single-stranded DNA may recombine with the host’s chromosome once inside the cell. This recombination replaces the gene in the host with a variant – albeit homologous – gene. DNA from a closely related genus may be acquired but, in general, DNA is not exchanged between distantly related microbes. Not all bacteria can become competent. Conjugation is a means of gene transfer in many species of bacteria.
Cell-to-cell contact by a specialized appendage, known as the F-pilus (or sex pilus), allows a copy of an F- plasmid (fertility plasmid) to transfer to Gift to Humans a cell that does not contain the plasmid. On rare occasions an F-plasmid may become integrated in the chromosome of its bacterial host, generating what is known as an Hfr (high frequency of on Relationships recombination) cell. Is A To Humans Essay? Such a cell can also direct the synthesis of a sex pilus. As the quotes chromosome of the Hfr cell replicates it may begin to cross the is a Gift to Humans Essay pilus so that plasmid and chromosomal DNA transfers to the recipient cell. And Crake Quotes? Such DNA may recombine with that of Gift to Humans Essay its new host, introducing new gene variants. Plasmids encoding genes for virulence factors antibiotic-resistance are passed throughout populations of bacteria, and Essay on Concurrent, between multiple species of bacteria by conjugation. In the laboratory, conjugation can be used to transfer disrupted genes on a self-transmissible plasmid, to develop a mutant strain. A gene of interest from a recipient E. coli strain is cloned into a self-transmissible vector and maintained in a donor strain for genetic manipulation. The deleted gene construct is then transferred by conjugation from the Gift donor strain back into the recipient strain. Transduction is another method for oryx and crake transferring genes from one bacterium to another; the transfer is mediated by bacteriophages. A bacteriophage infection starts when the virus injects its DNA into a bacterial cell.
The bacteriophage DNA may then direct the synthesis of Culture is a to Humans new viral components assembled in jefferson essay the bacterium. To Humans? Bacteriophage DNA is replicated and then packaged within the phage particles. Early in by Twain and Charles Dudley Warner Essay the infective cycle the phage encodes an enzyme that degrades the DNA of the host cell. Some of these fragments of bacterial DNA are packaged within the Culture is a Gift to Humans bacteriophage particles, taking the place of phage DNA. As the The Pros of Social Media number of phages increases it breaks open (lyse) the to Humans Essay cell. When released from the of the are consumer durable goods? infected cell, a phage that contains bacterial genes can continue to infect a new bacterial cell, transferring the bacterial genes. Sometimes genes transferred in this manner become integrated into the genome of their new bacterial host by homologous recombination. Such transduced bacteria are not lysed because they do not contain adequate phage DNA for viral synthesis and Gift, these phages can enter an alternate life cycle called lysogeny. In this cycle, all the virus’s DNA becomes integrated into the genome of the host bacterium. The integrated phage, called a prophage, can confer new properties to the bacterium. Researchers use transduction as a means of gene transfer as it requires a media like virus for transferring genes from one cell to the other.
Viruses are thus as a tool to introduce foreign DNA from the on Relationships selected species to the target organism. There are two types of transduction called as generalized transduction in which any of the bacterial gene is Culture to Humans, transferred via the bacteriophage to the other bacteria and specialized transduction involves transfer of limited or selected set of genes. Viral vectors for gene transfer: The use of Essay on Concurrent Disoders viruses as vectors for transduction, i.e. the introduction of genes into animal cells by exploiting the is a Essay natural ability of the virus particle, within which the oryx transgene is packaged, to adsorb to the surface of the cell and gain entry is being employed . Gift? Due to the efficiency with which viruses can deliver their nucleic acid into cells and the high levels of replication. and gene expression it is thomas, possible to achieve, viruses have been used as vectors not only for gene expression in cultured cells but also for gene transfer to living animals.
Four classes of viral vector have been developed for use in human gene therapy and have reached phase 1 clinical trials. These are the Culture Gift to Humans retrovirus, adenovirus, herpesvirus and adenoassociated virus (AAV) vectors . Before introducing the following are consumer durable individual vector systems,we discuss some general properties of viral transduction vectors. Culture Is A To Humans Essay? Transgenes may be incorporated into. viral vectors either by addition to the whole genome or by replacing one or more viral genes. This is generally achieved either by ligation (many viruses have been modified to incorporate unique restriction sites) or homologous recombination. For many applications, it is favourable to use vectors from which all viral coding sequences have been deleted.These amplicons (also described as ‘gutless vectors’) contain just the Essay example cis-acting elements required for packaging and genome replication. The advantage of such vectors is their high capacity for foreign DNA and the fact that, since no viral gene products are made, the vector has no intrinsic cytotoxic effects. The choice of vector depends on the particular properties of the virus and the intended host, whether transient or stable expression is is a Gift Essay, required and how much DNA needs to be packaged. For example, icosahedral viruses such as adenoviruses and oryx, retroviruses package their genomes into preformed capsids, whose volume defines the maximum amount of foreign DNA that can be accommodated. Conversely, rod-shaped viruses such as the baculoviruses form the capsid around the genome, so there are no such size constraints.
There is Culture, no ideal virus for gene transfer ‘ each has its own advantages and disadvantages. In recent years, a number of hybrid viral vectors have been developed incorporating the beneficial features of two or more viruses. 1) viral vectors that especially efficient at of the following are consumer durable goods? transducing the to Humans intended cell type but not other cell types. 2) retroviruses are particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 1) cannot deliver genes to terminally differentiated cells. 2) viral vectors may be highly immunogenic. TRANSFECTION BY CHEMICAL METHODS. One of the methods of gene transfer where the genetic material is deliberately introduced into the animal cell in view of studying various functions of proteins and the gene is transfection.
This mode of gene transfer involves creation of pores on the cell membrane enabling the cell to receive the foreign genetic material. When transformation is Essay on Concurrent Disoders, carried out in eukaryotic cells it is termed as transfection. 1) Calcium phosphate mediated DNA transfer. The process involves a mixture of isolated DNA (10-100ug) with solution of. calcium chloride and potassium phosphate. . When the two are combined, a fine precipitate of the positively charged calcium and the negatively charged phosphate will form, binding the DNA to be transfected on its surface .Cells are then incubated with precipitated DNA either in is a Essay solution or in Essay example tissue culture dish. A fraction of cells will take up the is a Gift Essay calcium phosphate DNA precipitate by quotes a process not entirely understood but thought to Culture is a Gift to Humans be endocytosis. Transfection efficiencies using calcium phosphate can be quite low, in the range of 1-2 % . Thomas? It can. be increased if very high purity DNA is used and the precipitate allowed to form slowly. 1. Frequency is very low.
2. Integrated genes undergo substantial modification. 3. Many cells do not like having the solid precipitate adhering to them. and the surface of Gift Essay their culture vessel. 4. Integration with host cell chromosome is random. 5, Due to above limitations transfection applied to somatic gene therapy.
This technique is used for introducing DNA into mammalian cells. This process has been a preferred method of identifying many oncogenes. 2)DNA transfer by DAE-Dextran method: DNA can be transferred with the help of and crake quotes DAE Dextran also. Culture To Humans Essay? DAE-Dextran may be used in the. transfection medium in Essay Disoders which DNA is is a to Humans, present. Oryx? This is a polycationic, high molecular weight substance and is a Essay, is convenient for transient assays. The negatively charged DNA binds to the polycation and the complex is taken up by and Cons of Social Media Essay the cell via endocytosis.
If DEAE (diethylaminoethyl )-Dextran treatment is coupled with Dimethyl Sulphoxide (DMSO) shock, then upto 80% transformed cell can express the transferred gene. The advantage of this method is that, it is cheap, simple and Culture is a Gift Essay, can be used for Essay transient cells. which cannot survive even short exposure of calcium phosphate. The major advantages of the technique are its relative simplicity and speed, limited expense, and remarkably reproducible interexperimental and intraexperimental transfection efficiency. Disadvantages include inhibition of cell growth and induction of heterogeneous morphological changes in Culture is a to Humans cells. Furthermore, the concentration of serum in the culture medium must be transiently reduced during the transfection. It does not appear to be efficient for the production of stable transfectants. For cells that grow in suspension, electroporation or lipofection is usually preferred, although DEAE-dextran-mediated transfection can be used. Transfer of DNA by polycation-DMSO: As calcium phosphate method of DNA transfer is reproducible and efficient but there is narrow range of optimum conditions.
DNA transfer by. Another polycationic chemical,the detergent Polybrene is carried out to increase the adsorption of DNA to the cell surface followed by a brief treatment with 25-30% DMSO to increase membrane permeability and enhance uptake of DNA. In this method no carrier DNA is required and oryx and crake, stable transformants are produced. This method works with mouse fibroblast and chick embryo. LIPOSOME MEDIATED GENE TRANSFER. Liposomes are spheres of lipids which can be used to transport molecules into the cells. These are artificial vesicles that can act as delivery agents for exogenous materials including transgenes.
They are considered as spheres of lipid bilayers surrounding the molecule to be transported and promote transport after fusing with the cell membrane. Liposomes for use as gene transfer vehicles are prepared by adding an appropriate mix of bilayer constituents to an aqueous solution of DNA molecules. In this aqueous environment, phospholipid hydrophilic heads associate with water while hydrophobic tails self-associate to exclude water from within the lipid bilayer. This self-organizing process creates discrete spheres of continuous lipid bilayer membrane enveloping a small quantity of DNA solution. The liposomes are then ready to be added to target cells. Germline transgenesis is possible with liposome mediated gene transfer.Lipofection is the most common and generally utilized gene transfer technique in the recent years and it utilizes cationic lipids. Is A Gift Essay? The combined DNA and cationic lipids act instantaneously to form structures called as lipoplexes that are more complex in structure than the jefferson essay simple liposomes. Cationic lipids have a positive charge and these form cationic liposomes which interact with the Culture to Humans Essay negatively charged cell membrane more readily than uncharged liposomes. This leads to a fusion between cationic liposome and Gilded Dudley, the cell surface resulting in quick entry by endocytosis and the delivery of the DNA directly across the plasma membrane.
Cationic liposomes can be produced from a number of cationic lipids that are commercially available and sold as an in vitro-transfecting agent, termed lipofectin. However this pathway would usually result in the fusion of lipoplexes with lysosomes and undergo degradation. This problem is overcome by utilizing the neutral helper lipids, which are generally included along with the cationic lipid. This allows entrapped DNA to Culture Essay escape the endosomes, reach the nucleus and get access to the cell’s transcriptional machinery.The endosomal structure is Essay on Concurrent Disoders, destroyed by Culture is a Gift to Humans increasing the osmotic pressure created by the lipids’ buffering action within the endosomes and by the fusion of the lipid with the thomas jefferson endosomal membrane. The ability of a lipid to is a Gift to Humans Essay destroy endosomes is one of the main characteristics of a transfection reagent. 2. And Cons Of Social Media Essay? Long term stability.
4. Protection of nucleic acid from Culture is a to Humans Essay degradation. 5. particularly proficient at Essay Disoders delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 6. cationic lipids effectively transfect suspension cells. 1) Cannot deliver genes to terminally differentiated cells. A rapid and simple technique for introducing genes into Culture is a Essay a wide variety of microbial, plant and animal cells, including E. Oryx? coli, is electroporation. Electroporation uses electrical pulse to produce transient pores in the plasma membrane thereby allowing macromolecules into the cells. These pores are known as electropores which allow the molecules, ions and water to pass from to Humans Essay one side of the membrane to another. The electropores reseal spontaneously and the cell can recover. The pores can be recovered only if a suitable electric pulse is and crake, applied .The formation of Culture Gift electropores depends upon the cells that are used and Essay on Concurrent, the amplitude and duration of the electric pulse that is applied to them. When subjected to electric shock, cells take up exogenous DNA from the suspending solution. Is A Gift To Humans Essay? Once inside the cell, the oryx and crake quotes DNA is integrated and the foreign gene will express.
A proportion of these cells become stably transformed and can be selected if a suitable marker gene is carried on the transforming DNA. Gift To Humans Essay? The most critical parameters are the intensity and duration of the electric pulse, and Age Coined by Mark Twain and Charles Essay, these must be determined empirically for different cell types. Electric currents can lead to dramatic heating of the cells that can results in cell death. Heating effects are minimized by using relatively high amplitude, a short duration pulse or by using two very short duration pulses. However, once optimal electroporation parameters have been established, the method is simple to carry out and highly reproducible. Many different factors affect the efficiency of Gift to Humans electroporation, including temperature, various electric-field parameters (voltage, resistance and capacitance), topological form of the DNA, and various host-cell factors (genetic background, growth conditions) and post-pulse treatment. General applications of electroporation. 1. Introduction of exogeneous DNA into animal cell lines, plant protoplast, yeast protoplast and bacterial protoplast. 2. Electroporation can be used to increase efficiency of transformation or transfection of bacterial cells. 3. Wheat, rice, maize, tobacco have been stably transformed with frequency upto 1% by this method.
4. Thomas Jefferson Essay? Genes encoding selectable marker may be used to Gift to Humans Essay introduce genes using electroporation. 5. Which Of The Following Are Consumer? To study the transient expression of molecular constructs. 6. Electroporation of early embryo may result in the production of Culture Essay transgenic animals. 7. Hepatocytes, epidermal cells, haematopoietic stem cells, fibroblast, mouse T and B lymphocytes can be transformed by this technique. 8. Naked DNA may be used for gene therapy by applying electroporation device on The Pros Media, animal cells. Advantages of electroporation. 1. Method is fast. 3. Applied for a number of cell types. 4. Simultaneously a large number of cell can be treated. 5. Gift To Humans Essay? High percentage of stable transformants can be produced.
1)Limited effective range of. 1 cm between the electrodes. 2)Surgical procedure is required to quotes place the electrodes deep into the internal organs. 3)High voltage applied to tissues can result in irreversible tissue damage as a result of thermal heating. The technique has high input costs, because a specialized capacitor discharge machine is required that can accurately control pulse length and amplitude.
4) Larger numbers of cells may be required than for other methods because, in many cases, the most efficient electroporation occurs when there is up to Culture is a to Humans 50% cell death. Sonication is the Essay example act of applying sound energy to agitate particles in a sample. Ultrasonic frequencies (20 kHz) are usually used, leading to the process also being known as ultrasonication . Sonoporation, or cellular sonication, is the use of these ultrasonic frequencies for modifying the permeability of the cell plasma membrane. Gift Essay? This technique is usually used in on Relationships Essay molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or transformation. Sonoporation employs the acoustic cavitation of microbubbles to enhance delivery of such large DNA molecules. Although the early results could achieve only 10- to Gift 30-fold increases in transfection efficiency in jefferson essay vitro, technical refinements have lead to enhancements of Culture is a Essay up to several thousand fold in vitro ,sufficient to encourage ultrasonication mediated gene transfer in vivo. 1) It is non-invasive and well tolerated, 2)extraordinary safety record over Media Essay, a wide range of frequency and intensity. 3)high levels of public acceptability and understanding.
there are highly sophisticated, flexible, cost-effective and readily available diagnostic and therapeutic systems that can achieve site-specific transfer of ultrasound energy almost anywhere in the body, except perhaps the lung. 4) The bioactivity of this technique is similar to, and in some cases found superior to, electroporation. 5) in vivo is relatively nontoxic. 1)Extended exposure to low-frequency (MHz) ultrasound has been demonstrated to Culture is a result in complete cellular death (rupturing) as well as microvascular hemorrhage and disruption of tissue structure, thus cellular viability must also be accounted for when employing this technique. 2) Whether ultrasound affects later steps in the transfection process, particularly plasmid entry into the nucleus, remains unclear. Sonoporation is and Cons of Social Media Essay, under active study for the introduction of foreign genes in tissue culture cells, especially mammalian cells.
Sonoporation is also being studied for use in targetedGene therapy in vivo, in a medical treatment scenario whereby a patient is given modified DNA, and an ultrasonic transducer might target this modified DNA into specific regions of the patient’s body. In microinjection DNA can be introduced into cells or protoplast with the help of very fine needles or. glass micropipettes having the diameter of 0.5 to 10 micrometer. The DNA may be directly delivered into the nucleus or in the cytoplasm. Some of the Culture to Humans Essay DNA injected may be taken up by the nucleus. Studies Example? The desired gene in the form of Culture is a to Humans plasmid or alone is injected directly into the plant protoplast. Injection into the nucleus is more efficient than that into the cytoplasm. Thomas Jefferson Essay? Micro-injection is carried out with automatic equipments (robotics) using a micro-needle. Computerized control of holding pipette, needle, microscope stage and video technology has improved the efficiency of this technique.
Advantages of microinjection. 1. Frequency of stable integration of Gift Essay DNA is far better as compare to other methods. 2. Method is quotes, effective in transforming primary cells as well as cells in established cultures. 3. The DNA injected in this process is subjected to Culture is a Gift less extensive modifications. 4. Mere precise integration of recombinant gene in limited copy number can be obtained.
2. Skilled personal required. 3.Has not been successful for many plant cells . 4. Embryonic cells preferred for manipulation. 5. Oryx? Knowledge of mating timing, oocyte recovery is essential. 6. Culture Is A Essay? Method is useful for protoplasts and not for the walled cells. 7. Process causes random integration. 8. Rearrangement or deletion of host DNA adjacent to site of integration are common.
9. The technique is laborious, technically difficult, and limited to the number of cells actually injected. Applications of microinjection. 1. Process is applicable for oryx and crake plant cell as well as animal cell but more common for animal cells. 2. Technique is ideally useful for producing transgenic animal quickly. 3. Procedure is important for gene transfer to embryonic cells. 4. Applied to inject DNA into plant nuclei. 5. Method has been successfully used with cells and protoplast of. tobacco, alfalfa etc. Fig: microinjection in mouse egg. Biolistics or particle bombardment is a physical method that uses accelerated microprojectiles to.
deliver DNA or other molecules into intact tissues and cells. Biolistics transformation is relatively new and novel method amongst the physical methods for artificial transfer of is a Gift to Humans exogenous DNA. The nucleic acid is of the following are consumer goods?, delivered through membrane penetration at a high velocity, usually connected to microprojectiles like microscopic particles of tungsten or gold coated into cells using an electrostatic pulse, air pressure, or gunpowder percussion. As the particles pass through the is a Gift cell, the DNA becomes free to integrate into the plant-cell genome. The gene gun is a device that literally fires DNA into target cells . Essay? The DNA to be transformed. into the cells is coated onto microscopic beads made of either gold or tungsten. Culture Gift To Humans? Beads are carefully. coated with DNA.
The coated beads are then attached to the end of the plastic bullet and loaded into. the firing chamber of the gene gun. An explosive force fires the bullet down the barrel of the gun. towards the target cells that lie just beyond the end of the barrel. When the bullet reaches the end of. the barrel it is caught and stopped, but the DNA coated beads continue on Media, toward the target cells. Some of the is a beads pass through the on Concurrent cell wall into the cytoplasm of the target cells.
Here the bead and. the DNA dissociate and Culture is a Essay, the cells become transformed. Essay Disoders? Once inside the Culture Gift target cells, the and Cons of Social Media Essay DNA is. solubilised and may be expressed. 1. Biolistics technique has been used successfully to transform soyabean, cotton, spruce, sugarcane, papaya, corn, sunflower, rice, maize, wheat, 2. Genomes of subcellular organelles have been accessible to genetic. manipulation by biolistic method. 3. Mitochondria of plants and chloroplast of chlamydomonas have been. 4. Method can be applied to filamentous fungi and yeast (mitochondria).
5. The particle gun has also been used with pollen, early stage. embryoids, meristems somatic embryos, plant cells, root section, seeds and pollen. 6. This approach has been shown to be effective for transferring transgenes into mammalian cells in Culture to Humans vivo . 1. Essay Example? Requirement of protoplast can be avoided. Gift To Humans Essay? Unlike electroporation and microinjection, this technique does not require protoplasts or even single-cell isolations and is applicable to even intact plant tissue. 2. Walled intact cells can be penetrated. 3. Manipulation of genome of subcellular organelles can be achieved. 4. This is also a highly mechanized or robotic mediated technique in which the. speed of the micro-projectile particles is controlled by foolproof mechanisms.
5) It is also gaining in use as a method for transferring DNA construct into whole animals. 6) This technique is fast, simple and safe, and it can transfer genes to a wide variety of tissues. 7)there appears to be no limits to the size or number of genes that can be delivered. 1. Integration is random. 2. Requirement of equipments. 3.It can be a challenge to obtain a sufficient number of cells modified by this method to see a biologically significant effect. A new technique is Essay on Concurrent, presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions.
At the site of the beam impact, due probably to local temperature changes, the Culture Essay cell membrane modifies its permeability. As a consequence of the hit, circular areas, whose radius may be apparently regulated by changing the irradiation time and/or the radiation intensity (energy), appear on the wall, last for a short time and fade spontaneously . It takes a fraction of following durable goods? a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. It is well established that a strong pressure wave, known as a laser-induced stress wave (LISW), accompanies laser-induced plasma. We have extended their method to deliver macromolecules, such as genes. Culture Is A Gift Essay? Plasmid DNA (circular DNA residing in thomas jefferson essay bacterial cytoplasm) is used as a vector of the gene of interest. We inject the plasmid into target tissue, on which a laser target is placed, and Culture is a Gift to Humans Essay, subject the target to irradiation with a high-intensity, nanosecond laser pulse to induce plasma and The Pros Media, hence an Culture is a LISW. By placing optically transparent material on the target, the plasma is confined, resulting in an increase in the LISW’s impulse.
Interaction of tissue cells with the The Pros LISW allows plasmid to Culture Gift enter the cytoplasm. 1) it only takes advantage of the Essay on Concurrent Disoders presence of Culture Essay phenol-red, a normal cell medium component, with no need of addition of extraneous substances; (2) it is Age Coined by, a very mild treatment virtually suitable for any cell type and. (3) it allows transfection of is a to Humans Essay selected cells even in Studies example the presence of cells of different type (providing that they are morphologically distinguishable). 4) . it is rapidly replacing virus mediated techniques as they have serious side effects caused by immune response and limited targeting characteristics rendering them inappropriate for clinical application. 5) The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude. 6) . It enables minimally invasive tissue interaction. Thus there are a number of ways by which the genes can be introduced into the cells. With the advent of molecular tools and technologies it is now comparatively easy to introduce gene into cells without losing its integrity and biological activity. Culture Is A Gift Essay? Moreover the recent development in molecular biology has made the transfer of gene with great accuracy to the target cell. Which Of The Are Consumer Durable? The transfer of gene through different gene transfer technologies has cured a number of Gift to Humans Essay diseases. Research is on progress to quotes cure those diseases which cannot be cured by Culture is a Gift using drugs.
Moreover the The Pros treatment of diseases by is a to Humans gene transfer provides better result for a prolong period of time. It is the need of hour to discover new and cheap method of gene transfer technologies so to make the treatment of the diseases a little easier and affordable. Improvements in gene transfer are required in terms of transfection approaches to allow improved transgene uptake efficiencies. 1)Society of Applied Sciences. Gene Transfer Technologies and their Applications: K.H.KHAN.
Medical Biotechnology Division, School of Essay on Concurrent Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India. 2)Principles of gene manipulation: R.W.Old ,S.B. Primrose and R.M.Twymann ‘ sixth edition,chapter 10 and 11. 3) BIOTECHNOLOGY 2020-From the Transparent Cell to. the Custom-Designed Process–Prof. Gerhard Kreysa Dr.-Ing. E.h. Dr.h.c.
Dr. R??diger Marquardt. 4) INTRODUCTION TOBIOTECHNOLOGY AND GENETIC ENGINEERING. 5)From genes to genomes- concepts and applications of DNA technology–Jeremy W Dale, Malcom von Schantz. Search our thousands of to Humans Essay essays: If this essay isn't quite what you're looking for, why not order your own custom Science essay, dissertation or piece of coursework that answers your exact question? There are UK writers just like me on hand, waiting to help you. Each of us is qualified to a high level in our area of expertise, and oryx and crake quotes, we can write you a fully researched, fully referenced complete original answer to your essay question. Just complete our simple order form and you could have your customised Science work in your email box, in as little as 3 hours. This Science essay was submitted to us by a student in Culture is a Gift to Humans Essay order to help you with your studies. This page has approximately words.
If you use part of this page in your own work, you need to provide a citation, as follows: Essay UK, Essay: Gene transfer techniques – pros and cons . Available from: http://www.essay.uk.com/essays/science/essay-gene-transfer-techniques-pros-and-cons/ [05-10-17]. If you are the original author of this content and by Twain and Charles Warner, no longer wish to Culture is a have it published on our website then please click on the link below to request removal: 13-09-17 - The Human Genome project (HGP) 22-08-17 - Adeno associated virus (AAV) vectors 01-08-17 - Test methods on the alkali carbonate reaction in on Relationships example concrete - fluxonic diode - Identifying an unknown organism from a cell phone 05-07-17 - Risk factors for alcohol abuse in men who have sex with men 01-06-17 - Monsanto vs. the world 19-05-17 - Patterns formed by increasing the scale factor – maths 12-05-17 - Swine influenza infections 24-04-17 - Medicinal plants and wound healing. We offer professional custom essay writing, dissertation writing and coursework writing service.
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14 Skills and Values Employers Seek in Jobseekers. by Randall S. Hansen, Ph.D., and Katharine Hansen, Ph.D. Job Skills to list on your Resume. Deals with acting in a responsible and fair manner in all your personal and work activities, which is seen as a sign of maturity and self-confidence; avoid being petty. How to describe this skill on your resume: Conscientious go-getter who is highly organized, dedicated, and committed to is a to Humans Essay, professionalism. Employers probably respect personal integrity more than any other value, especially in light of the many recent corporate scandals. How to describe this skill on your resume: Seasoned professional whose honesty and integrity create effective leadership and optimal business relationships.
Deals with openness to new ideas and concepts, to working independently or as part of a team, and to carrying out which of the are consumer durable multiple tasks or projects. Is A? How to describe this skill on your resume: Highly adaptable, mobile, positive, resilient, patient risk-taker who is open to new ideas. Employers seek jobseekers who love what they do and will keep at it until they solve the problem and get the job done. How to Essay Disoders, describe this skill on your resume: Productive worker with solid work ethic who exerts optimal effort in successfully completing tasks. Culture Is A To Humans Essay? 5.Dependability/Reliability/Responsibility.
There#8217;s no question that all employers desire employees who will arrive to work every day? on time? and ready to work, and who will take responsibility for their actions. How to describe this skill on your resume: Dependable, responsible contributor committed to excellence and success. Essay Example? Employers want employees who will have a strong devotion to the company? even at times when the company is not necessarily loyal to its employees. How to Culture is a, describe this skill on your resume: Loyal and dedicated manager with an goods? excellent work record. 7.Positive Attitude/Motivation/Energy/Passion. The jobseekers who get hired and the employees who get promoted are the ones with drive and passion? and who demonstrate this enthusiasm through their words and actions. Is A Gift? How to describe this skill on your resume: Energetic performer consistently cited for oryx quotes unbridled passion for work, sunny disposition, and upbeat, positive attitude. Look at it this way: if you don#8217;t believe in yourself, in your unique mix of skills, education, and abilities, why should a prospective employer? Be confident in yourself and what you can offer employers. How to describe this skill on your resume: Confident, hard-working employee who is committed to achieving excellence.
9.Self-Motivated/Ability to Work Without Supervision. To Humans? While teamwork is always mentioned as an on Concurrent Disoders important skill, so is the ability to work independently, with minimal supervision. Is A? How to describe this skill on your resume: Highly motivated self-starter who takes initiative with minimal supervision. No matter what your age, no matter how much experience you have, you should always be willing to learn a new skill or technique. Jobs are constantly changing and evolving, and you must show an openness to and crake, grow and learn with that change.
How to Culture is a to Humans, describe this skill on your resume: Enthusiastic, knowledge-hungry learner, eager to meet challenges and quickly assimilate new concepts. Studies Essay? While there is some debate about whether leadership is something people are born with, these skills deal with your ability to is a to Humans, take charge and Essay on Concurrent Disoders manage your co-workers. How to describe this skill on Culture is a to Humans Essay, your resume: Goal-driven leader who maintains a productive climate and confidently motivates, mobilizes, and coaches employees to meet high-performance standards. 12.Multicultural Sensitivity/Awareness. There is possibly no bigger issue in the workplace than diversity, and jobseekers must demonstrate a sensitivity and awareness to other people and cultures. Which Of The Are Consumer Durable Goods?? How to is a to Humans Essay, describe this skill on your resume: Personable professional whose strengths include cultural sensitivity and of the goods? an ability to build rapport with a diverse workforce in multicultural settings. Deals with your ability to design, plan, organize, and implement projects and tasks within an allotted timeframe. Also, involves goal-setting. How to describe this skill on your resume: Results-driven achiever with exemplary planning and organizational skills, along with a high degree of Culture Gift Essay detail orientation.
Because so many jobs involve working in thomas, one or more work-groups, you must have the ability to work with others in a professional manner while attempting to achieve a common goal. How to describe this skill on your resume: Resourceful team player who excels at building trusting relationships with customers and Gift to Humans colleagues. Final Thoughts on Employment Skills and Values. Employability skills and personal values are the critical tools and traits you need to succeed in the workplace? and and Cons of Social Media they are all elements that you can learn, cultivate, develop, and maintain over your lifetime. Once you have identified the sought-after skills and values and to Humans Essay assessed the degree to which you possess them, begin to market them by building them into your resume, cover letter, and interview answers) for job-search success.
See also our Transferable Job Skills for Jobseekers.Click here to begin building your own resume! More Information about Employability Skills: Skills Employers Seek, reporting on annual results from the National Association of Colleges and Employers (NACE) survey of employers to determine the top 10 personal qualities/skills employers seek. Twain Essay? From the Career Development Center at Binghamton University. Skills Employers Seek, from Loughborough University. Skills Employers Seek, from Psych Web Top 10 Soft Skills in Demand, from LiveCareer Resume Skills Section, from LiveCareer.
Building Tools That Build Better Work Lives. Since 2005, LiveCareer’s team of career coaches, certified resume writers, and savvy technologists have been developing career tools that have helped over 10 million users build stronger resumes, write more persuasive cover letters, and develop better interview skills. Use our free samples, templates, and writing guides and our easy-to-use resume builder software to help land the job you want. Dr. Randall S. Hansen. Dr. Randall S. Hansen is Culture is a to Humans Essay founder of Quintessential Careers, one of the oldest and most comprehensive career development sites on the Web, as well CEO of thomas jefferson EmpoweringSites.com.
He is Culture is a Gift to Humans also founder of MyCollegeSuccessStory.com and EnhanceMyVocabulary.com. He is oryx quotes publisher of Culture Gift to Humans Essay Quintessential Careers Press, including the Quintessential Careers electronic newsletter, QuintZine. Dr. Hansen is also a published author, with several books, chapters in books, and of the goods? hundreds of articles. He’s often quoted in the media and conducts empowering workshops around the country. Finally, Dr. Hansen is also an educator, having taught at the college level for more than 15 years. Visit his personal Website or reach him by is a Gift email at firstname.lastname@example.org. Check out Dr.
Hansen on GooglePlus. Katharine Hansen, Ph.D., creative director and which associate publisher of is a Gift Quintessential Careers, is an educator, author, and blogger who provides content for Quintessential Careers, edits QuintZine, an electronic newsletter for jobseekers, and blogs about by Mark Twain and Charles Dudley Warner storytelling in the job search at A Storied Career. To Humans Essay? Katharine, who earned her PhD in organizational behavior from Union Institute University, Cincinnati, OH, is author of Dynamic Cover Letters for New Graduates and A Foot in the Door: Networking Your Way into on Concurrent, the Hidden Job Market (both published by Ten Speed Press), as well as Top Notch Executive Resumes (Career Press); and with Randall S. Hansen, Ph.D., Dynamic Cover Letters, Write Your Way to Culture Essay, a Higher GPA (Ten Speed), and The Complete Idiot’s Guide to Study Skills (Alpha). Visit her personal Website or reach her by e-mail at Gilded by Mark Twain and Charles Dudley email@example.com. Check out Dr. Hansen on GooglePlus.
I AM A CAREER CHANGER This page is your key source for Culture is a Gift all things career-change related. You#8217;ll find some great free career-change tools and resources. Changing careers can be traumatic, especially if you have been in your current career for a long time, but you do not have to which of the following are consumer goods?, go through the Culture Essay process alone or  Quintessential Careers: Career and Job-Hunting Blog. Quintessential Careers: Career and Job-Hunting Blog Career and job-search news, trends, and scoops for job-seekers, compiled by the staff of Quintessential Careers.The Quintessential Careers Blog has moved!! These pages remain as an archive of our previous blog posts. Please check out the new and improved Quintessential Careers Blog for Job-Seekers and Careerists.
Interview Advice Job  The Quintessential Directory of Company Career Centers. The Quintessential Directory of Company Career Centers Where job-seekers can go directly to the job/career/employment section of a specific employer#8217;s Website.Because more and more companies are developing career and employment centers on following, their corporate Websites, Quintessential Careers has developed this directory, which allows you to go straight to Culture is a Essay, the career and employment section of the  Quintessential Careers: I am a Career Coach or Counselor. The Quintessential Directory of Company Career Centers Where job-seekers can go directly to the job/career/employment section of a specific employer#8217;s Website.Because more and more companies are developing career and employment centers on their corporate Websites, Quintessential Careers has developed this directory, which allows you to go straight to on Relationships, the career and employment section of the  Mighty Recruiter Mighty Recruiter. Customer Service Customer Service. 800-652-8430 Mon- Fri 8am - 8pm CST.
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Examples Of Muet Report Writing Essays and Culture is a Gift Research Papers. some time building contacts and relationships that will help them in their chosen career. Report writing evaluation . Content – 25 Clear and concise background Selection of relevant information Analysis of information collected Substantial and well-researched data Achievement report objectives Language 30 Grammatically accurate Appropriate vocabulary Appropriate language for report writing Originality of Age Coined by Twain and Charles Essay, text production Visual aids 10 Accuracy of data and is a Gift Essay clarity of information . 2010 , Leisure , Personal life 465 Words | 3 Pages. CUSTOMER SATISFACTION REPORT BASED ON THE LATEST NATIONAL PASSENGER SURVEY (SPRING 2011) AUTHOR: ONUR AYAN DATE: 27/10/2011 TO: MANAGERS . Thomas Essay! OF SOUTH WEST TRAINS Summary In this report we have analysed the results of National Passenger Survey. The research gives us vital information about Culture, our customers’ opinions on our services.
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Attacking Faulty Reasoning , Critical thinking , Fallacy 1005 Words | 3 Pages. ?Book Report Writing Template This is a guide only. Jefferson Essay! It is Culture is a Gift to Humans not meant to write the report for you. It gives you a . format or template for Studies on Relationships example writing your own report . The original draft was developed by is a Gift to Humans Essay, a teacher friend of mine to use with her own students. I have made a few additional enhancements which I believe make the quotes model more clear and Essay complete. A note of caution here. Thomas Essay! Your instructor may have another book report format that he/she prefers. So, make sure they approve of this format before.
Character , Protagonist , Typography 934 Words | 4 Pages. purpose for Culture Gift to Humans this hole is to make the joint or connection by using the and crake screw. As we know, the joint between the part is Culture Gift Essay very important for the machine . Oryx Quotes! rigidity. Is A! A hole is needs to transfer the flow of the of the durable liquid that use in this machine. For example , the Gift to Humans liquid used for and crake quotes the coolant will be transfer by a pipe that need the hole for Gift Essay this piping system.
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Essay , Report , Technical communication 580 Words | 7 Pages. A Guide to Essay Disoders, Writing a Reflective Report. A Guide to Writing a Reflective Report What does it mean ‘to reflect’? Officially, it means to explore experiences in order to . Culture Is A Gift To Humans! lead to thomas, new understandings and improved practice. Culture Gift To Humans Essay! At its simplest it means: • To think deeply about an experience. Gilded By Twain And Charles Dudley Warner Essay! To go beyond the simple question, ‘What’s going on Culture Gift Essay, here?’ to ask ‘What’s really going on here?’ • To ask yourself what this experience means to you and your practice • To churn ideas, thoughts and on Concurrent experiences around in your head and make connections . Experience , Mind , Reflection 1324 Words | 5 Pages. All reports need to be clear, concise and Culture to Humans well structured.
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A Guide to Writing a Reflective Report. A guide to writing a Reflective Report The purpose of the Reflective Report is to foster an ability to reflect on . their experience and consider ways in which their developing understanding of the and crake theory, which underpins practice, can help them to develop an attitude of critical awareness of their own actions, values, motives etc, and also to the actions, values and motivations of others that they work with. Culture! Students complete a weekly reflective report during level 1, and a fortnightly reflective. Critical theory , Critical thinking , Reflection 1290 Words | 4 Pages. Academic Writing Analysis Example. the audience (i.e. Studies Essay! tutor) against Culture is a Gift human cloning and expresses the author’s lack of competency in presenting his arguments. Based on the channel chosen . to convey his message, the essay is, to a large extent, an ineffective piece of academic writing upon evaluation of on Concurrent Disoders, its content, language and organization of arguments. This essay critique analyses and evaluates the above points. One of the essay problems includes the poorly developed thesis statement at the end of the Culture 1st paragraph.
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Communication , Writer , Writing 2449 Words | 7 Pages. Guidelines For Writing Training Report. ?Guidelines For Writing Training Report The Guidelines for Culture to Humans Essay carrying out the of the following are consumer durable STR is given in to Humans Essay, the following paragraphs. Each . student is to compile his/her study in six chapters as detailed below: Chapter-I : Introduction 1.1 Introduction: It should include (a) Meaning of the concept, ie, Job Satisfaction, Consumer Satisfaction, Working Capital Management, (b) Rationale for choosing the Essay on Concurrent Disoders topic/problem under study, (c) Implementation strategy of concept in your present study. 1.2 Objectives of Study. Data , Data analysis , Exploratory data analysis 906 Words | 3 Pages.
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Annual report , Balance sheet , Business 1705 Words | 6 Pages. Definitions of technical writing 1. The goal of technical writing is to enable readers to use a technology or understand a . Is A Gift To Humans! process or concept. Because the subject matter is more important than the are consumer writer's voice, technical writing style uses an is a objective, not a subjective, tone. The writing style is direct and utilitarian, emphasizing exactness and clarity rather than elegance or allusiveness. A technical writer uses figurative language only when a figure of speech would facilitate understanding. Communication , Technical communication , Technical writer 468 Words | 3 Pages. ?Recommendation Report Of evaluate office products to improve working environment To: Ellen Page From: Roger Liao Date: November 2.2014 . Summary: Smash Design decide to Gilded Age Coined Mark Twain and Charles Warner Essay, purchase new chairs and some ergonomic items to Gift to Humans Essay, solve the Media problem of to Humans, employees feel uncomfortable and get sick in their work environment. This report evaluate the Herman Miller Chairs and Studies on Relationships Essay example Steel Case Leap Chairs, Simple Package and Deluxe Package offer by Staple supplies, and Rocco and Rita’s Gym membership. Aeron Chairs with higher. Aeron chair , Chair , Chairs 906 Words | 4 Pages. Trial Report Writing for Industrial Attachment.
SHORT REPORTS PURPOSE: When a brief written communication is necessary and can do the job for the reader. ? For . example , a weekly or monthly financial or production report can be handled by a Short Report . ? Or, a proposal for a simple project or improvement on a process can be written up in a Short Report . WHAT IS THE FORM? Can be written as a Letter or as a Memo. ? Memo when it is written within your department or company. (Sometimes a letter is Culture is a Essay OK here.) ? Letter when. Memorandum , Page , Pages 860 Words | 4 Pages. Writing Unit Folio Case Study Report. TOTAL Intern: Alexandra Carmichael Andrece: Final Case Study Report for Unit Writing Folio Teacher: Mrs. Kathy Jacobs . Grade: 3rd Blanche Fuqua Elementary School DEMOGRAPHICS SHEET Name: Alex Carmichael Teacher: Kathy Jacobs School: Blanche Fuqua Elementary Directions: Place “X” by all descriptors that apply to your school.
Community: _____Rural _____Suburban ___X__ Urban _____Other _____Mostly affluent _____Mostly upper middle-class families . Colon , Full stop , Orthography 1140 Words | 4 Pages. TIPS FOR MUET CANDIDATES! Try not to treat or think of MUET as a scary test. However, don't under estimate the preparation, . time and effort required for this test either. If you have friends or classmates who have taken the test, ask them about it. You'll probably get many many different types of thomas essay, answers. OMG!
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Technical writing is Essay on Concurrent a form of technical communication used in a variety of technical and occupational fields, such as computer . hardware and software, engineering, chemistry, aeronautics and astronautics, robotics, finance, consumer electronics, andbiotechnology. The Society for Culture is a Technical Communication (STC) defines technical writing  as a broad field including any form of communication that exhibits one or more of the following characteristics: (1) communicating about technical or specialized. Communication , Documentation , Help authoring tool 970 Words | 3 Pages. Executive – composition and function, the Gilded Age Coined by Mark Dudley Essay role of the Civil Service. c. To Humans! Judiciary – Structure of the court system, functions of Magistrates’ Courts, High . Courts, Privy Council, Caribbean Court of Justice d. Role of the security systems, for example , police service, prison service. Functions of government: a. Raise revenue through direct and jefferson essay indirect taxation. b. Provide social services: welfare, education, health, public utilities. Is A! c. Manage the finances of the Studies on Relationships Essay example country (budgeting). Gift! d. Maintain. Democracy , Election , Elections 698 Words | 3 Pages. ?Anjali Patel Riley Egan, Nick Morrison 10/2/13 Block 2 LAB REPORT OUTLINE FOR DIFFUSION/OSMOSIS LAB Introduction: Diffusion is the net . movement of small or uncharged molecules or substances from are consumer durable goods?, areas of Culture Gift, higher concentration to areas of jefferson, lower concentration. Gift To Humans! There is oryx and crake quotes simple diffusion, as explained, and facilitated diffusion. Gift To Humans! Facilitated diffusion, on the other hand, involves larger or charged molecules or substances that need a protein tunnel through the membrane to help the substance enter. Chemistry , Concentration , Diffusion 768 Words | 4 Pages.
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Business terms , Costs , Finance lease 1659 Words | 7 Pages. XXXXXXXXX Exploratory Report Stake-Holders: People/Entities The conflict of the The Pros and Cons of Social Media Essay Ground Zero mosque has become one of the Culture is a to Humans Essay fiercest religious . based debates in recent years. One of the Media Essay most influential men in the debate is Feisal Abdul Rauf. Rauf is the Imam (leader in prayer) at the current New York City mosque just a few blocks away from the proposed location of the is a Gift future mosque. He has put a lot of time and Gilded by Mark Twain Warner Essay money into this proposed mosque and does not plan to give up on it. Another key person in. Barack Obama , Democratic Party , Hillary Rodham Clinton 977 Words | 3 Pages. A Written Report Presented to MR. MARK GIL GABE Department of Social Sciences and Humanities College of Education and Gift Essay Social Sciences . Mindanao State University at Naawan Misamis Oriental In partial Fulfillment Of the quotes Requirements for Culture is a Gift to Humans the Course English 8 (Technical Writing ) Elmer Alcano Progress Report DATE : MAY 20 2013 TO : Mr. Mark Gil M. Gabe FROM : Elmer Alcano SUBJECT.
Abbas Kiarostami , Alphabet , Cagayan de Oro City 707 Words | 3 Pages. of report writing in Business Organizations need accurate, timely, objective and concise information to make effective . decisions. On Concurrent! One way they can obtain such information is from a business report . This document can be defined as “an organizedpresentation of information to a specific audience for the purpose of Culture is a to Humans, helping an organization achieve an which are consumer goods? objective” While business reports share similar characteristics, they also need to be customized for their particular purpose. An informal report may. Advertising , Corporation , Creative writing 1601 Words | 6 Pages. Progress Phase Report : Phase 1 MKTG304 – Friday 10am Andrew West Table of Contents Introduction . . 2 Consumption Patterns Type of Gift Essay, trends, fluctuations and cycles in demand . Studies On Relationships Essay Example! 2 Consumer . Consumer , Consumer protection , Dishwasher 2100 Words | 7 Pages. Expository Writing What is it?
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In many respects, scribbling is the fountainhead for writing and Culture Gift Essay occurs. Creative writing , Fiction writing , Literature 856 Words | 3 Pages.